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  1. #1
    TY Concentrates Mentor Graywolf's Avatar

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    Default Growing cannabis from tissue culture

    Here are some purdy ladies grown from stem cells in a special medium by our inhouse red headed geek, instead of seeds or clones. The stem cells are extracted from the stem nodes anywhere on the mother plant.

    One of the advantages is that no diseases or systemic parasites are passed on.

    Hee, hee, hee, snicker, snark, snort.....................
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  2. #2
    Flowering Member Guy_In_Pain's Avatar

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    GW how big are the tissue cultures when they start out? Are they recognizeable to the naked eye?

    There is a future in this for any patient that has to follow plant numbers so keeping strains alive for future use is paramount. Where I am they deem a plant to be anything with roots so this is an excellent work around to keep genetics going without interfering with plant count.

    And one last question, does it take a long time to begin to grow and look like cannabis, what about root growth?

    Thanks for the post and always for the reply.

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    Vegetative Member schmade420's Avatar

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    So fricking cool...I've been reading as much info on tissue culture as i can possibly find. Such a revolutionary concept being able to have thousands of strains on a shelf.

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    Data Nazi Lightly_Toasted's Avatar

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    Most definately interesting. I would like to know more on the subject as well

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    TY Concentrates Mentor Graywolf's Avatar

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    Quote Originally Posted by Guy_In_Pain
    GW how big are the tissue cultures when they start out? Are they recognizeable to the naked eye?

    There is a future in this for any patient that has to follow plant numbers so keeping strains alive for future use is paramount. Where I am they deem a plant to be anything with roots so this is an excellent work around to keep genetics going without interfering with plant count.

    And one last question, does it take a long time to begin to grow and look like cannabis, what about root growth?

    Thanks for the post and always for the reply.
    The meristem tissue culture starts are about a millimeter slice through a branch node. You can see them with the naked eye, but they are not recognizable as anything but a thin sliver of some kind of plant material.

    The foliage that you see in the picture took less than a week in a nutrient solution with hormones and growth regulators.

    It has to be put in a different solution to get it to root. It will never root in the current solution, just keep uptting off more harvestable starts.

    You're welcome!

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    TY Concentrates Mentor Graywolf's Avatar

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    Quote Originally Posted by lightly_toasted
    Most definately interesting. I would like to know more on the subject as well

    I'll keep ya'll updated on progress and it the whole thing works out, Growgeek promises to share the details and do a video.

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    Seedling Spuzzum's Avatar

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    Back in the 90's, High Times had an article of something "similar".. researcher scraped the younger, soft stems, then put the plant fibers/material into a container with rooting gel. The fibers eventually rooted and created new shoots.

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    Seedling rxb's Avatar

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    The tobacco industry has been playing with this for a while now and has some pretty good research available although it's pretty technical so very dry reading.

    I'd be a little worried I cross contaminated something and ended up making the blob that ate L.A.... not that I'd miss L.A. really...

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    Vegetative Member Dr Dave's Avatar

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    Quote Originally Posted by rxb
    I'd be a little worried I cross contaminated something and ended up making the blob that ate L.A.... not that I'd miss L.A. really...

    Lol, no one would miss it, plus I bet we could all band together and hunt down the giant cannabis blob and have free meds for years, woot!
    Thanks to google and youtube I managed to fabricate all the above words together to sound like Im growing, but Im not, never have, and even with a MMAR card I never will, so there! P.S. I loves my prozac and ridalin, om nom nom, moar plz....

  10. #10
    Seedling rxb's Avatar

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    Quote Originally Posted by Dr Dave
    Lol, no one would miss it, plus I bet we could all band together and hunt down the giant cannabis blob and have free meds for years, woot!
    Herd it into moonshine country and make Lake QWISO... I like it

  11. #11
    Data Nazi Lightly_Toasted's Avatar

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    My thoughts are to what other plants I could apply this to. I do a lot of plant rescues in my freetime, would be a possible way to save some that are in bad shape. Not to say that I won't master it and use it for cannabis as well

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    Vegetative Member

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    Same procedure as orchids, I'd guess.

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    Data Nazi Lightly_Toasted's Avatar

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    I actually have read up on cloning orchids, most of what I found made for a very dry read and my brain just didn't want to absorb it. I seem to be a magnetfor sick orchids, had 18 at one point. Sad thing is they are actually very picky, but simple to care for. Maybe I shall attempt another read on the subject.

  14. #14
    Theraputic Cannabis Dominatrix pflover's Avatar

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    awesome, is it possible to by the culture solution premade or do you have to create it yourself?
    Regestered PDX area TC-caregiver for 2, housewife.

    Mugen: [after finding a bag of coins in the river] We hit the pot, Jack!
    Fuu: That's jackpot!
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    Fuu: Filthy rich!
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    PRESERVE NEURAL PLASTICITY!!!

    Anit harm none...

  15. #15
    Seedling rxb's Avatar

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    you can get pre made and there are even all inclusive kits available. Just google "plant tissue culture" or add the word "kit" and there are tons of products. --r

  16. #16
    carl
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    Default this is exciting stuff

    i really like your posts graywolf and i would like to speak at length to you directly

  17. #17
    inkog77
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    are there any books about the subject ...

    cresting tissue sample to form full grown plants

  18. #18
    inkog77
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    sorry bx just deleted mail.. been having problems with mail box in ty..... couple of time......s

    ,,, it been ongoing problem..

    peace

  19. #19
    Vegetative Member Dr Dave's Avatar

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    i wonder a couple things... first how long does a tissue sample take to grow into a plant ready to veg, and two, can these samples be stored dormant without growing?
    Thanks to google and youtube I managed to fabricate all the above words together to sound like Im growing, but Im not, never have, and even with a MMAR card I never will, so there! P.S. I loves my prozac and ridalin, om nom nom, moar plz....

  20. #20
    TY Concentrates Mentor Graywolf's Avatar

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    Quote Originally Posted by lightly_toasted
    My thoughts are to what other plants I could apply this to. I do a lot of plant rescues in my freetime, would be a possible way to save some that are in bad shape. Not to say that I won't master it and use it for cannabis as well
    GG is using the techniques and culture solutions developed for Orchids and African Violet, which he also does. I'm not knowledgeable on what plants can and cannot be succeffully cultured this way, but I suspect most of them.

  21. #21
    TY Concentrates Mentor Graywolf's Avatar

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    Quote Originally Posted by pflover
    awesome, is it possible to by the culture solution premade or do you have to create it yourself?

    GG is buying both his culture mediums premade. Everything is simple and easy, though extreme measures are necessary keep everything sterile. So far he has lost only one culture solution to bread mold, but mold is the bete noir of culturing.

  22. #22
    TY Concentrates Mentor Graywolf's Avatar

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    Quote Originally Posted by rxb
    you can get pre made and there are even all inclusive kits available. Just google "plant tissue culture" or add the word "kit" and there are tons of products. --r
    True! You can also find the same process information GG found by Googling cannabis micro propogation protocol.

  23. #23
    TY Concentrates Mentor Graywolf's Avatar

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    Quote Originally Posted by Dr Dave
    i wonder a couple things... first how long does a tissue sample take to grow into a plant ready to veg, and two, can these samples be stored dormant without growing?
    Since this is the first experiment GG has done with cannabis, I really don't know the answer yet. So far I can tell you that the meristematic tissue literally explodes with foliage once in the culture medium. Attached is the latest picture of a culture put up 3-18, so it is right at two weeks old. As you can see, it is ready to either take cuttings from or move it to a larger container.

    It can also be moved to the rooting culture at this stage.
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    Last edited by Graywolf; 04-05-2011 at 07:07 AM.

  24. #24
    inkog77
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    looking good

    can you freeze like dooms day then use this shit to go ape...

  25. #25
    TY Concentrates Mentor Graywolf's Avatar

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    Quote Originally Posted by inkog77
    looking good

    can you freeze like dooms day then use this shit to go ape...

    Don't know! Maybe if it was fast frozen so as to not rupture the cells with ice crystals.

    I added trying a liquid nitrogen freeze, followed by tissue culturing to the project list.

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