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  1. #1
    Seedling Havic's Avatar

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    Arrow A citrus tree with THC

    Florida Biochemist designs a citrus tree with THC

    In the summer of 1984, 10th-grader Irwin Nanofsky and a friend were driving down the Apalachee Parkway on the way home from baseball practice when they were pulled over by a police officer for a minor traffic infraction.


    After Nanofsky produced his driver’s license the police officer asked permission to search the vehicle. In less than two minutes, the officer found a homemade pipe underneath the passenger’s seat of the Ford Aerostar belonging to the teenage driver’s parents. The minivan was seized, and the two youths were taken into custody on suspicion of drug possession.

    Illegal possession of drug paraphernalia ranks second only to open container violations on the crime blotter of this Florida college town. And yet the routine arrest of 16 year-old Nanofsky and the seizure of his family’s minivan would inspire one of the most controversial drug-related scientific discoveries of the century.

    Meet Hugo Nanofsky, biochemist, Florida State University tenured professor, and the parental authority who posted bail for Irwin Nanofsky the night of July 8, 1984. The elder Nanofsky wasn’t pleased that his son had been arrested for possession of drug paraphernalia, and he became livid when Tallahassee police informed him that the Aerostar minivan would be permanently remanded to police custody.

    Over the course of the next three weeks, Nanofsky penned dozens of irate letters to the local police chief, the Tallahassee City Council, the State District Attorney and, finally, even to area newspapers. But it was all to no avail.

    Under advisement of the family lawyer, Irwin Nanofsky pled guilty to possession of drug paraphernalia in order to receive a suspended sentence and have his juvenile court record sealed. But in doing so, the family minivan became “an accessory to the crime.” According to Florida State law, it also became the property of the Tallahassee Police Department Drug Task Force. In time, the adult Nanofsky would learn that there was nothing he could do legally to wrest the vehicle from the hands of the state.

    It was in the fall of 1984 that the John Chapman Professor of Biochemistry at Florida State University, now driving to work behind the wheel of a used Pontiac Bonneville, first set on a pet project that he hoped would “dissolve irrational legislation with a solid dose of reason.” Nanofsky knew he would never get his family’s car back, but he had plans to make sure that no one else would be pulled through the gears of what he considers a Kafka-esque drug enforcement bureaucracy.

    “It’s quite simple, really,” Nanofsky explains, “I wanted to combine Citrus sinesis with Delta 9-tetrahydrocannabinol.” In layman’s terms, the respected college professor proposed to grow oranges that would contain THC, the active ingredient in marijuana. Fourteen years later, that project is complete, and Nanofsky has succeeded where his letter writing campaign of yore failed: he has the undivided attention of the nation’s top drug enforcement agencies, political figures, and media outlets.

    The turning point in the Nanofsky saga came when the straight-laced professor posted a message to Internet newsgroups announcing that he was offering “cannabis-equivalent orange tree seeds” at no cost via the U.S. mail. Several weeks later, U.S. Justice Department officials showed up at the mailing address used in the Internet announcement: a tiny office on the second floor of the Dittmer Laboratory of Chemistry building on the FSU campus. There they would wait for another 40 minutes before Prof. Nanofsky finished delivering a lecture to graduate students on his recent research into the “cis-trans photoisomerization of olefins.”

    “I knew it was only a matter of time before someone sent me more than just a self-addressed stamped envelope,” Nanofsky quips, “but I was surprised to see Janet Reno’s special assistant at my door.” After a series of closed door discussions, Nanofsky agreed to cease distribution of the THC-orange seeds until the legal status of the possibly narcotic plant species is established.

    Much to the chagrin of authorities, the effort to regulate Nanofsky’s invention may be too little too late. Several hundred packets containing 40 to 50 seeds each have already been sent to those who’ve requested them, and Nanofsky is not obliged to produce his mailing records. Under current law, no crime has been committed and it is unlikely that charges will be brought against the fruit’s inventor.

    Now it is federal authorities who must confront the nation’s unwieldy body of inconsistent drug laws. According to a source at the Drug Enforcement Agency, it may be months if not years before all the issues involved are sorted out, leaving a gaping hole in U.S. drug policy in the meantime. At the heart of the confusion is the fact that THC now naturally occurs in a new species of citrus fruit.

    As policy analysts and hemp advocates alike have been quick to point out, the apparent legality (for now) of Nanofsky’s “pot orange” may render debates over the legalization of marijuana moot. In fact, Florida’s top law enforcement officials admit that even if the cultivation of Nanofsky’s orange were to be outlawed, it would be exceedingly difficult to identify the presence of outlawed fruit among the state’s largest agricultural crop.

    Amidst all of the hubbub surrounding his father’s experiment, Irwin Nanofsky exudes calm indifference. Now 30-years-old and a successful environmental photographer, the younger Nanofsky can’t understand what all of the fuss is about. “My dad’s a chemist. He makes polymers. I doubt it ever crossed his mind that as a result of his work tomorrow’s kids will be able to get high off of half an orange.”

    Biochem 101: How to design a Cannabis-equivalent citrus plant

    Step One:
    Biochemically isolate all the required enzymes for the production of THC.

    Step Two:
    Perform N-terminal sequencing on isolated enzymes, design degenerate PCR (polymerase chain reaction) primers and amplify the genes.

    Step Three:
    Clone genes into an agrobacterial vector by introducing the desired piece of DNA into a plasmid containing a transfer or T-DNA. The mixture is transformed into Agrobacterium tumefaciens, a gram negative bacterium.

    Step Four:
    Use the Agrobacterium tumefaciens to infect citrus plants after wounding. The transfer DNA will proceed to host cells by a mechanism similar to conjugation. The DNA is randomly integrated into the host genome and will be inherited.

    Any reports of it in the wild?

    --------------------------------------------------------------------------------------------------------Man I would Love to get my hands on some of them seeds!

    http://thecrit.com/2008/10/05/florid...tree-with-thc/
    Last edited by Havic; 12-13-2008 at 02:11 PM.
    THE OLD CODE
    Be without fear in the face of your enemies; be brave, just and upright so God may love thee. Speak the truth always. Do no harm, Safeguard the helpless.

    *Marijuana, in its natural form, is one of the safest therapeutically active substances known. In strict medical terms, marijuana is safer than many foods we commonly consume.
    -Judge Francis Young

    "The Emperor Wears No Clothes" Entire book online at: http://www.onlinepot.org/grow/jackherer.htm

  2. #2
    R.I.P. No Longer Suffering Swampy's Avatar

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    you can call me OJ if you feed me those kinda oranges
    We are all related, brothers and sisters to all that walk, crawl, swim and fly, cousins to the dust and rain that refresh our Mother Earth. Always remember life loves you and hate is never the answer

  3. #3
    Zygote

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    Default Great experiment by a great man. Revenge is as sweet as a orange

    I am quite happy and enterained to learn this.I ahve definetly added this professor to the list of professors i would like to meet and quite please to see someone else turn a tragerdy in to a triumph for the medical commuity and majority of simple users. I would like to know more of how he did the process to do this and how to get hold of the professor as a fellow horticultirist. The technique is worth more than the seeds it self as it can help with alot of crops and their healt. We have all know from the recent test on bacteria and virus that thc slows down the progress of these plagues. Ideas on how to use for medical application have been on the table for a long time now. Recent studies have seen that test of thc on MRSA has been quite sucessful in slowing and reducing without tolerance by the virus. Hopefully someone has this professors contact information that ic an get hold of him with visit to the states. I should be in that region.

    The sequencing is cool but to know which sequence and do it correctly is not easy and to make sure it remains stable in the corss without affecting other genes i think is tricky.

    Thanks for the info

    Bless
    Last edited by Rainmakker; 12-13-2008 at 02:33 PM.

  4. #4
    Seedling neuroherb's Avatar

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    Great story, need to do some searching on the seeds as it wouldn't take much for the folk wo already have them to create many more.

  5. #5
    Retired Staff

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    This is crazy! I would love one of those trees! I would like to meet this profeesor as well. He seems to be right up my alley, i am studying to be a bio-med enginner. and plant genetics seems to be all the rage lately.

  6. #6
    Seedling Havic's Avatar

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    Quote Originally Posted by neuroherb
    Great story, need to do some searching on the seeds as it wouldn't take much for the folk wo already have them to create many more.
    If anyone comes up with more info plz post. Comments are always welcome too.
    THE OLD CODE
    Be without fear in the face of your enemies; be brave, just and upright so God may love thee. Speak the truth always. Do no harm, Safeguard the helpless.

    *Marijuana, in its natural form, is one of the safest therapeutically active substances known. In strict medical terms, marijuana is safer than many foods we commonly consume.
    -Judge Francis Young

    "The Emperor Wears No Clothes" Entire book online at: http://www.onlinepot.org/grow/jackherer.htm

  7. #7
    Vegetative Member RatherBBurnin's Avatar

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    ITS FAKE!!!!

    Its been passed around canna sites for the last couple years.. its not true.. its a total hoax..... I cant remember where they found the link to say it was fake at CSC.. but this has been floating around the internet for sometime... and isnt legit..


    sorry folks...

  8. #8
    Seedling neuroherb's Avatar

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    Did seem a bit like and April 1st news item, but would be cool if it was done and there sure is the tech in the mainstream biology & botany folks to do the job.

  9. #9
    R.I.P. No Longer Suffering Swampy's Avatar

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    lol yep too good to be true but can you imagine marmalade hash
    We are all related, brothers and sisters to all that walk, crawl, swim and fly, cousins to the dust and rain that refresh our Mother Earth. Always remember life loves you and hate is never the answer

  10. #10
    Theraputic Cannabis Dominatrix pflover's Avatar

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    if only it were that simple.

    love the "laughing man" avtr, havic.
    Regestered PDX area TC-caregiver for 2, housewife.

    Mugen: [after finding a bag of coins in the river] We hit the pot, Jack!
    Fuu: That's jackpot!
    Mugen: All I know is we're filthy bitch!
    Fuu: Filthy rich!
    Jin: I hope your doing that on purpose.

    PRESERVE NEURAL PLASTICITY!!!

    Anit harm none...

  11. #11
    Embryo still_buzz'n's Avatar

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    Yes...it's total bullshiite! Sure wish I could get a few of those beans to work with...lol!
    "Please do not put your cigarette butts in our urinals for we do not piss in your ashtrays!"
    -My Dad

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